Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC EDTA P10-15100 6X100ML
PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Apexbio Technology LLC One-step TUNEL Cy3 Apoptosis Detection Kit 50rxns
One-step TUNEL Cy3 Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation occurring in tissues or cells during apoptosis Cellular DNA endonucleases are activated during apoptosis which cleave genomic DNA between nucleosomes yielding DNA fragments of approximately 180 - 200 bp or their multiples The resulting DNA fragments have exposed 3 -OH ends which can be labeled with a fluorescein-modified dUTP under the action of Terminal Deoxynucleotidyl Transferase (TdT) These labeled DNA fragments can then be detected using fluorescence microscopy or flow cytometry (Ex/Em) of 550 nm/570 nm This detection kit has a wide range of applications and can be used to assay apoptosis in frozen sections or paraffin-embedded tissue sections as well as cultured adherent or suspended cells
Alkali Scientific Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 25mL, 25/Case
Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 25mL, 25/Case. This 25mL bottle contains a special formula designed to neutralize disinfectants while enriching microbial samples. Its sterile condition ensures no contamination, and the ready-to-use format reduces preparation time, making it an ideal solution for microbiological laboratories. Perfect for researchers requiring neutralization of disinfectants.
Boston Bioproducts Inc Saline-Sodium Citrate (20X) - 4L
Saline-Sodium Citrate (20X) - 4L (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ 1X Balanced Salt Solution, 500ml, 10/Case
Balanced salt solution for cell culture applications. All formulations incorporate a rigorous specification and approval process. Available in lot sizes that facilitate scale-up and manufacturing.
Biotang Inc Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml
Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml.
Apexbio Technology LLC Annexin V-PE/DAPI Apoptosis Kit 20tests
Annexin V-PE/DAPI Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit includes the nuclear stain DAPI which specifically binds to double-stranded DNA to emit blue fluorescence Normally DAPI cannot penetrate intact cell membranes In late apoptotic and dead cells DAPI can cross the compromised membrane to stain the nucleus
Apexbio Technology LLC TUNEL Apoptosis Detection Kit (DAB) 20rxns
The TUNEL Apoptosis Detection Kit (DAB) is designed to detect nuclear DNA fragmentation occurring during apoptosis in tissues or cells During apoptosis endonucleases within cells become activated and cleave genomic DNA between nucleosomes resulting in DNA fragments of approximately 180-200 bp or multiples thereof The fragmented DNA generates free 3 -OH termini at which terminal deoxynucleotidyl transferase (TdT) incorporates biotin-labeled dUTP Subsequently these labeled termini bind to horseradish peroxidase (HRP)-conjugated streptavidin (Streptavidin-HRP) followed by chromogenic visualization using DAB This kit allows direct observation using an ordinary optical microscope and is convenient for use It is applicable to a wide range of sample types including frozen sections paraffin-embedded sections adherent cell cultures and suspended cells making it versatile for apoptosis detection
Bioworld Carbonate-buffered saline pH 9.5 (5X), 250 mL
This buffer contains 125mM sodium carbonate and 750mM sodium chloride, pH 9.5. The buffer is important for several different cellular processes. It is one of the major buffering systems used to maintain the pH of mammalian blood and is also important in the acid-base homeostasis of living things, including humans. It tends to maintain a relatively constant plasma pH and counteract any force that would alter it.Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption. Appearance: LiquidPH Value: 9.5
ILEX LIFE SCIENCES LLC TRYPSIN P10-021500 500ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (500 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Teknova 1X PBS Solution, pH 7.4. 100mL, Sterile. 10 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
