Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-25.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4) - 500ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). It is not soluble in water unless pH is adjusted accordingly. EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers.
Teknova 1M HEPES, pH 6.8. 1000mL, Sterile 2 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-100.0.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-50.03.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 50 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-5000.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-1000.010.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 1 Kilogram
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Alkali Scientific CellPro™ Tris-HCL Buffer 1M, pH 7.5, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ TAE 50X (Tris-Acetate EDTA), 2L
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Alkali Scientific CellPro™ Tris Base, 1M, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
![Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B32070-25.02.JPG-250.jpg)
Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 25 Grams
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-1000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 1 Kilogram
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-100.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 100 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9